In goat milk, several bands were observed, the band before [alpha]-LAC was of low molecular weight proteins and might be
proteose and peptones.
Axenic cultures of Tetrahymena pyriformis were obtained from Carolina Biological Supply Company (Carolina) and maintained in Carolina's growth medium (
proteose peptone, 5 g/l; tryptone, 5 g/l; [K.sub.2]HP[O.sub.4], 1.1 mmol/l).
unicolor), 0.1% Tween 80, and 0.05%
proteose peptone was used for mutanase production.
Bacteria were cultured at 18-20[degrees]C in modified King's B ([KB.sup.*]) medium (20 g
proteose peptone (Oxoid, UK), 10 g glycerol, 1.5 g [K.sub.2]HP[O.sub.4], and 1.5 g MgS[O.sub.4] per litre with 1.5% (w/v) agar added for plates) and maintained at -80[degrees]C as 15% (w/v) glycerol stocks.
Similarly, to study their effect on xylanase production, different nitrogen sources (both organic and inorganic) like yeast extract, urea, soybean meal,
proteose peptone, [(N[H.sub.4]).sub.2]S[O.sub.4], KN[O.sub.3], NaN[O.sub.3] (Hi Media Pvt.
These incubated cultures were added to a modified Duncan and Strong medium (
proteose pepton 15.0 g, yeast extract 4.0 g, sodium thioglycolate 1.0 g, sodium phosphate 10.0 g, raffinose 4.0 g, 0.51 mM caffein 50.0 mL in 1 L of distilled water) to obtain 5% concentration of the culture and incubated in an anaerobic jar at 37[degrees]C for 48 h.
Whey proteins are approximately 20% of the milk proteins and comprised of [beta]-lactoglobulin (50%), [alpha]-lactalbumin (20%), bovine serum albumin (10%) and other minor proteins (10%) like lactoferrin, peptone, immunoglobins, prolactin,
proteose, folate, calmodulin and binding proteins.
The water sample was then serially diluted with sterile saline and spread onto Reasoner's 2A agar (0.5 g/L
proteose; 0.5 g/L casamino acids; 0.5 g/L yeast extract; 0.5 g/L dextrose; 0.5 g/L soluble starch; 0.3 g/L dipotassium phosphate; 0.05 g/L magnesium sulfate; and 0.3 g/L sodium pyruvate) and incubated overnight (24 h) at 28[degrees]C.
The peptides were originally isolated from the sequence of bovine milk proteins: 5 residues peptide from [alpha]-lactalbumin was named E-5-K, 16 residues peptide from [alpha]-s2 casein was named L-16-Y, and 23 residues peptide from component-3 of
proteose peptone (PP3) was named N-23-T or lactophoricin-I [32] (the latter is alternatively denoted in the literature as LPcin-I [40, 41]).
The Staphylococcus strains were grown on medium Manitol Salt Agar (MSA) consisting of
Proteose peptone 5g\L, Sodium chloride 75g\L, and Mannitol 10g\L, Phenol red 0.025 g\L and Agar 15g.
Figure 1: Whey Proteins are the Healthy Constituent of Milk WHEY PROTEINS [beta]-lactoglobulin 35% [alpha]-lactalbumin 12% glycomacropeptide 12%
proteose peptone 12% immunoglobulins 8% serum albumin 5% lactoferrin 1% lactoperoxidase 0.5% minor proteins 15% CASEIN [alpha]s1-, [alpha]s2-, [beta], [kappa] casein The protein component of milk is composed of 20% whey and 80% casein.
Briefly, the media was biphasic, consisting of Bacto agar with
Proteose Peptone No.