genipap

Genipap (Genipa Americana L.) is a soft brown berry fruit widely distributed in tropical Central and South America (Porto et al., 2014).

Here, we propose a suitable process for extraction and purification of polyphenol and genipin compounds from genipap fruit pulp.

Ripe genipap fruit were purchased from a local market (Uberlandia, state Minas Gerais, Brazil).

Genipap fruits were manually washed and sanitized (10 ppm sodium hypochlorite).

(Rubiaceae), popularly known as genipap has an economic importance, both for its forestry and environmental potential (DURAES et al., 2014).

Application of tissue culture techniques works as a tool to overcome propagation problems of species such as genipap. These techniques use not only enables the maximization of production of seedlings with genetic fidelity, but also ensures the conservation of germplasm (REED et al., 2011).

It is possible to obtain in vitro genipap regeneration from the conversion of zygotic embryos on MS medium supplemented with 4.44 [micro]M BAP and NAA.

Among these plants are genipap, which contains compounds including genipic acids, secoiridoids, tannins, and genipapin (GOTTLIEB & MORS, 1980; ABRAO, 2010); barn, which contains triterpenes, isoflavones, and phenolic compounds (PUEBLA et al., 2010); and taruma, which contains butanolid and tarumal (SANTOS et al., 2001; FONSECA et al., 2006).

Genipap was collected from November 2013 to January 2014 in the central region of the city, bara from August to September 2013, and taruma in December 2013 on the campus of the Universidade Federal do Mato Grosso do Sul.

The results of the disc diffusion tests for the hydroalcoholic extracts are listed in table 1 (genipap), table 2 (bara), and table 3 (taruma).

In the disc diffusion tests with genipap, the 30% pulp extract displayed the largest inhibition zones against the four microorganisms tested.