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The BMSCs were labeled with DiI (1,1'-dioctadecyl-3,3,3'3'- tetra methyl in do carbo cyanine perchlorate) (Sigma, China) in the ovaries to show the presence of the transplanted cells after 4 weeks.
Silver and cyanine staining of oligonucleotides in polyacrylamide gel.
Starting with 400 ng of total RNA, cyanine 3 (Cy3)-labeled cRNA was produced according to the manufacturer's protocol.
(c) PFP, platelet-free plasma; PRP, platelet-rich plasma; Cy5, cyanine 5.
Currently, several categories of probes have been studied, which generally include organic NIR dyes such as cyanine dyes, rhodamine dyes, BODIPY-based NIR probes, squaraine-based NIR probes, phthalocyanines and porphyrin derivatives, and nanoprobes such as NIR dye-containing nonmetallic nanoparticles, gold nanostructures, and quantum dots.[111]
DOs were kept in Cyanine Dyes 3-conjugated goat-rabbit immunoglobulin G (1:200, Abcam) for an hour, washed thoroughly, then transferred into 1 [micro]g/mL DAPI for 10 min.
Shahedi, Novel Ag/Kaolin Nanocomposite as Adsorbent for Removal of Acid Cyanine 5R from Aqueous Solution, J.
The antibodies were conjugated to fluorescein (FITC), phycoerythrin (PE), or cyanine 3 (Cy3).
It is based on the well-recognized ability of aluminum to form colourful complexes with organic compounds such as eriochrome cyanine R (3"-sulfo-3,3'-dimethyl-4-hydroxyfuchson-5,5'-dicarboxylic (ECR)) (Figure 1), catechol violet [5], or aluminon [6].
Our group has recently developed a new experimental methodology for the identification of G-quadruplex forming sequences using the cyanine dye Thiazole Orange (TO).
Briefly, seamless high-resolution images were acquired using an 8-bit monochrome TDI line-image capture camera with filters specific for DAPI to define the nuclear compartment, either fluorescein isothiocyanate (FITC) or Cyanine 3 (Cy3) to define the vimentin-positive tumor cytosolic compartment, and Cy5 to define all target markers.